Journal: Leukemia
Article Title: HDAC7 is a potential therapeutic target in acute erythroid leukemia
doi: 10.1038/s41375-024-02394-5
Figure Lengend Snippet: A Experimental scheme used in ( B – D ). Human cord blood (CB) CD34 + cells were transduced with p53DD (coexpressing NGFR) and ERG (coexpressing GFP) and cultured in the presence of EPO. B Proliferation curves of CB cells transduced with vector, p53DD, ERG, or p53DD+ERG for 4 days. Results are expressed as mean ± s.e.m. of three independent experiments. **** P < 0.0001. CD71 and CD235a expression ( C ) and Wright-Giemsa-staining ( D ), original magnification x400) of CB cells transduced with vector, p53DD, ERG, or p53DD+ERG. E CD235a expression of F36P, HEL, and TF-1 cells transduced with vector or ERG.
Article Snippet: CEP53 cells were cultured in RPMI-1640 medium supplemented with 10% FBS containing 1 ng/ml EPO (#959-ME, R&D Systems).
Techniques: Transduction, Cell Culture, Plasmid Preparation, Expressing, Staining